Gradient LC-MS determination of 22 cardiovascular drugs of several classes (beta blockers, potassium-sparing diuretics, statins, nucleoside transport inhibitors, calcium channel blockers, ACE inhibitors, ARBs, lipid-lowering agents and anticoagulants) on an ASTRA® C18-HE 3.0 µm column, 75 mm × 2.1 mm, part number AST-5732-IH21. Mobile phase A is DDW + 0.1% formic acid and mobile phase B is ACN + 0.1% formic acid. Gradient elution (time / A% / B%): 0 / 90 / 10, 1 / 90 / 10, 4 / 75 / 25, 8 / 60 / 40, 10 / 0 / 100, 14 / 0 / 100, 14.01 / 90 / 10, 15 / 90 / 10, 17 / 90 / 10. Flow rate 0.35 mL/min, column temperature 23 °C, injection volume 5 µL, detection by full scan (resolving power 120 000 at m/z 200) in positive mode. Analytes with CAS numbers as printed: 1. atenolol 29122-68-7,…