Antidiabetic-drugs
INTRODUCTION
Diabetes is a chronic condition that occurs when the body cannot produce any or enough insulin, or cannot effectively use the insulin it produces. Type 2 diabetes has attained the status of a global pandemic. Sulfonylureas represent a class of medications used in the treatment of type 2 diabetes mellitus. All sulfonylureas contain a phenyl-sulfonyl-urea structure, which exerts the hypoglycemic effect. An efficient and reliable LC/MS method has been developed for сontrol of antidiabetic medications of sulfonylurea class glibenclamide and glimepiride.
ABSTRACT
LC-MS full-scan determination of two sulfonylurea antidiabetic drugs, glibenclamide and glimepiride, on an ASTRA® C18-HE 3 µm column, 75 mm × 2.1 mm, part number AST-5732-IH21. Mobile phase A is DDW + 0.1% formic acid and mobile phase B is ACN + 0.1% formic acid. Gradient elution (time / A% / B%): 0 / 90 / 10, 1 / 90 / 10, 4 / 75 / 25, 8 / 60 / 40, 10 / 0 / 100, 14 / 0 / 100, 14.01 / 90 / 10, 15 / 90 / 10, 17 / 90 / 1 (final B percentage as printed in the note). Flow rate 0.35 mL/min, column temperature 23 °C, injection volume 5 µL, detection by full scan (resolving power 120 000 at m/z 200) in positive mode. Analytes with CAS numbers as printed: 1. glibenclamide (glyburide), CAS number 10238-21-8, 2. glimepiride, CAS number 261361-60-8. The note shows two extracted…
Industries
Pharma & Veterinary Drugs
Category
HPLC columns
Product
Astra C18-HE
Part number
AST-5732-IH21
Analytes
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- Glibenclamide (glyburide) CAS number 10238-21-8
- Glimepiride CAS number 261361-60-8
