Pesticides play a critical role in food protection against pathogens and pests. However, pesticides pose significant risks to non-target organisms, including animals and humans. The number of pesticides is continuously growing as new active substances are being developed. For this reason, a robust and reliable method for simultaneous determination of multiple analytes, is very relevant today. ASTRA® Pesticides column is designed for routine environmental analysis and provides reliable retention under high-aqueous conditions for wide class of compounds including triazines, chlorophenoxy acids, organophosphates, fungicides and herbicide metabolites.
ABSTRACT
Quick and robust LC-MS/MS method for the simultaneous determination of residual concentrations of pesticides and their isomers in water matrices, with Micro-Solid-Phase-Extraction (MSPE) used for sample clean-up. The analysis is performed on an ASTRA® Pesticides 3 µm column, 50 mm × 2.1 mm, part number AST-6015-IG21 (mass spectrometer TSQ Quantiva). Mobile phase A is DDW + 0.1% formic acid and mobile phase B is ACN + 0.1% formic acid. Gradient elution (time / A% / B% / flow µL/min): 0 / 100 / 0 / 300, 1 / 100 / 0 / 300, 4 / 60 / 40 / 350, 6 / 20 / 80 / 400, 9 / 0 / 100 / 400, 10 / 0 / 100 / 400, 10.1 / 100 / 0 / 300, 13 / 100 / 0 / 300. Column temperature 30 °C, injection volume 10 µL, detection HESI-MRM in positive/negative ion mode. MSPE clean-up uses an HLB 0.22 µm PTFE…
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Atrazine-desethylCAS number not available
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Partners
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Faculty of Fisheries and Protection of Waters
Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, Laboratory of Environmental Chemistry and Biochemistry. Named in APP NOTE 533 as the co-operating partner for the MSPE and LC-HRMS determination of long-chain PFASs in fish biota.
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