Estrogens analysis
INTRODUCTION
Analysis of endogenous estrogens is challenging due to the low physiological concentrations and weak UV absorption. Multiple metabolites with similar chemical structures and close molecular weights as well as co-eluting substances from biological fluids may cause significant ion suppression in mass spectrometry. Ionization of estrogens is inefficient, introduction of derivatization agents may enhance ionization, but non-specific product ions can be generated. Therefore, the method generating derivatives with product ions specific to individual estrogens is highly demanded.
ABSTRACT
Quick and robust LC-MS/MS method for the analysis of the two naturally occurring estrogens estrone (E1) and estradiol (E2), analysed as derivatives, on an ASTRA® C18-Hybrid 5 µm column, 50 mm × 4.6 mm, part number AST-6016-LG46. Mobile phase 70% MeOH + 10% 50 mM (NH4)2CO3 + 20% UPW; the detailed gradient table and the LC-MS conditions are declared proprietary in the note and are therefore not disclosed. Flow rate 1 mL/min, column temperature 50 °C, injection volume 60 µL. Analytes: 1. estrone (E1), CAS number 53-16-7, 2. estradiol (E2), CAS number 50-28-2. The note is built around robustness and selectivity comparisons, all showing E1 in the upper and E2 in the lower chromatograms: derivatized E1/E2 in a standard mixture and in a plasma sample; the plasma sample on two different…
Industries
Clinical & Toxicology
Category
HPLC columns
Product
Astra C18-Hybrid
Part number
AST-6016-LG46
Analytes
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- Estrone (E1) CAS number 53-16-7
- Estradiol (E2) CAS number 50-28-2
