Vitamin D
INTRODUCTION
Vitamin D is a group of steroids that have various effects on the human body and support the immune system. This application shows LC/MS/MS separation of the hydroxylated and non-hydroxylated forms of vitamin D2 and vitamin D3. The amount of their hydroxylated forms determines the total vitamin D in a blood sample. Non-hydroxylated forms of vitamin D2 and vitamin D3 are important for food analysis. The LC/MS/MS chromatogram shows the separation of all the above mentioned analytes.
ABSTRACT
LC/MS/MS determination of 25-OH-vitamin D2 (CAS 21343-40-8), 25-OH-vitamin D3 (CAS 19356-17-3), vitamin D2 (CAS 50-14-6) and vitamin D3 (CAS 67-97-0) in a dry blood spot, on an ARION® Plus C18, 2.2 µm column (100 mm × 2.1 mm, ARI-5720-EI21). Gradient elution with A: H2O + 0,1 % formic acid and B: MeOH + 0,1 % formic acid at 0.4 ml/min, 25 °C, injection volume 10 µl, UV detection at 280 nm. MS conditions: positive APCI, nitrogen as collision gas, MRM transitions 413.32 → 395.30 (25-OH-D2), 401.22 → 365.40 (25-OH-D3), 397.44 → 379.20 (D2) and 385.32 → 259.40 (D3), dwell time 150 ms. The hydroxylated pair elutes around 5.3 min and the non-hydroxylated forms between 8.5 and 9 min. This application was developed by Ján Šmoldas.
Industries
Pharma & Veterinary Drugs
Category
HPLC columns
Product
Arion Plus C18
Part number
ARI-5720-EI21
Analytes
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- 25-OH-Vitamin D3 CAS number 19356-17-3
- 25-OH-Vitamin D2 CAS number 21343-40-8
- Vitamin D2 CAS number 50-14-6
- Vitamin D3 CAS number 67-97-0
