Alcaloids_Xanthine_derivatives
INTRODUCTION
Xanthine alkaloids occur naturally in various plants, such as cocoa, tea and coffee tree. They are commonly used for their effects as mild stimulants. Xanthine alkaloids are monitored in food and drinks, e.g. in chocolate, cocoa powder, and energy drinks.
ABSTRACT
Simultaneous determination of theobromine (CAS 83-67-2) and caffeine (CAS 58-08-2) on an ARION® Plus C18, 5.0 µm column (250 mm × 4.6 mm, ARI-5720-LM46). Isocratic elution with methanol/water 30/70 (v/v) at 1.0 ml/min and 30 °C, UV detection at 280 nm. Both analytes are baseline separated within 10 minutes; the application shows a standard, a cocoa sample and an energy drink sample.
Industries
Category
HPLC columns
Product
Arion Plus C18
Part number
ARI-5720-LM46
Analytes
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- Theobromine CAS number 83-67-2
- Caffeine CAS number 58-08-2
