Oligonucleotides II

INTRODUCTION

ARION® Polar C18 shows much better retention and resolution in GC 52 % oligonucleotides analysis than competitive column developed for this particulate application.

ABSTRACT

Analysis of a 100 µM oligonucleotide with 25 bases on an ARION® Polar C18, 5 µm column (250 mm × 4.6 mm, ARI-5721-LM46). Gradient elution with TEAA (100 mM) : ACN/methanol (1:1) 90/10 (v/v) at 1.1 ml/min and 55 °C, UV detection at 260 nm. The ARION® Polar C18 retains the product longer (about 9 minutes against 6.7 minutes on the competitive column) and resolves it better from the neighbouring shorter sequences. This application has been developed by GENERI BIOTECH, certified EU-located company.

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Industries

Product

Arion Polar C18

Part number

ARI-5721-LM46

Analytes

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Oligonucleotide with 25 bases CAS number not available

Partners

GENERI BIOTECH

Czech biotechnology company producing synthetic oligonucleotides, fluorescent probes and molecular diagnostic kits, with its own quality control of every synthesised sequence.

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