This application note has been developed by the Institute of Organic Chemistry and Biochemistry, the group of synthesis of radiolabeled compounds. It shows separation of nucleosides, and nucleoside phosphates that are analysed in different applications, e.g. purine metabolism, biosynthesis, genetic code and clinical studies.
ABSTRACT
Separation of nucleosides and nucleoside phosphates – IMP (CAS 131-99-7), AMP (CAS 61-19-8), inosine (CAS 58-63-9), ADP (CAS 58-64-0), ATP (CAS 56-65-5) and adenosine (CAS 58-61-7) – on an ARION® Plus C18, 5.0 µm column (250 mm × 4.6 mm, ARI-5720-LM46). Gradient elution with A: water + 2 M TEAB (5 %) and B: water : acetonitrile (50 : 50 v/v) + 2 M TEAB (5 %) at 1.0 ml/min and 25 °C, injection volume 6 µl, PDA 2998 detection at 254 nm on a Waters Alliance e2695 system (Empower 3 Pro). All six compounds are resolved within 16 minutes.
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IMP (Inosine monophosphate)CAS number 131-99-7
AMP (Adenosine monophosphate)CAS number 61-19-8
InosineCAS number 58-63-9
ADP (Adenosine diphosphate)CAS number 58-64-0
ATP (Adenosine triphosphate)CAS number 56-65-5
ADO (Adenosine)CAS number 58-61-7
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IOCB Prague – Institute of Organic Chemistry and Biochemistry of the CAS
Research institute of the Czech Academy of Sciences in Prague working in organic chemistry, biochemistry and medicinal chemistry. The group of synthesis of radiolabeled compounds prepares labelled nucleosides and nucleotides.
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