Tramadol_LCMS
INTRODUCTION
Tramadol is a centrally acting opioid analgesic belonging to the pharmacotherapeutic group of opioid analgesic. Tramadol is sometimes used in combination with Paracetamol to treat moderate to severe pain suffered by patients with various types of pain. This application has been developed to monitor right drug dosing.
ABSTRACT
LC/MS/MS determination of tramadol (CAS 2914-77-4) and its metabolites O-desmethyltramadol (CAS 73986-53-5) and N-desmethyltramadol (CAS 75377-45-6), with tramadol-D3 as internal standard, on an ARION® Plus C18, 3 µm column (50 mm × 3.0 mm, ARI-5720-IG30). Gradient elution with A: 1 % ammonium formate in water and B: methanol / 1 % ammonium formate in ACN 50/50 (v/v) at 0.5 ml/min, autosampler 15 °C and column oven 30 °C, injection 2.0 µl, detection on a Sciex QTRAP 4500 with an ExionLC™ system. O-desmethyltramadol elutes at about 3.0 min, tramadol and N-desmethyltramadol at about 3.4 min; each compound has a quantifier and a qualifier transition at 35 ± 15 eV.
Industries
Clinical & Toxicology
Category
HPLC columns
Product
Arion Plus C18
Part number
ARI-5720-IG30
Analytes
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- O-Desmethyltramadol CAS number 73986-53-5
- N-Desmethyltramadol CAS number 75377-45-6
- Tramadol CAS number 2914-77-4
