Amines is a group of compounds, which is not easy to separate. The Novel ASTRA® DM phase with a polar embeded group and polar selector enables the separation of different amines and organic compounds with an amino-functional group. Peaks show an excellent symmetry under shown conditions.
ABSTRACT
Separation of amines and aromatic compounds – kynurenine (CAS 343-65-7, D/L), tryptamine (CAS 61-54-1), acetophenone (CAS 98-86-2), melatonine (CAS 73-31-4), toluene (CAS 108-88-3) and naphthalene (CAS 91-20-3) – on an ASTRA® DM, 5 µm column (150 mm × 4.6 mm, AST-5810-LK46). Isocratic elution with A: 100 mM DEA + 100 mM FA, pH 3.0 and B: methanol in the ratio 15:85 (v/v) at 0.75 ml/min and 20 °C, injection volume 1.0 µl, UV detection at 250 nm. The polar-embedded group and polar selector of the DM phase retain the amino-functional compounds and give symmetrical peaks across a 12-minute run.
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KynurenineCAS number 343-65-7
TryptamineCAS number 61-54-1
AcetophenoneCAS number 98-86-2
MelatonineCAS number 73-31-4
TolueneCAS number 108-88-3
NaphthaleneCAS number 91-20-3
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