The group of water-soluble B vitamins is widely monitored in dietary supplements and in food. They play an important role in cell metabolism and the synthesis of red blood cells. This application shows the HPLC separation of B1, B2, B3, B6 and B12 vitamins.
ABSTRACT
HPLC method for the separation of the water-soluble B-group vitamins monitored in dietary supplements and food. The separation is carried out on an ARION(R) Plus C18 column, 3 um, 100 mm x 4.6 mm, part number ARI-5720-II46. The mobile phase is A: 0.0125 M phosphate buffer (pH 3.5) and B: methanol, run as a gradient (time / %A / %B): 0 / 95 / 5, 3 / 95 / 5, 10 / 30 / 70, 10.5 / 30 / 70. Flow rate 1.0 ml/min, column temperature 40 C, injection volume 5.0 ul, UV detection at 280 nm. Five analytes are separated within about 9 minutes and elute in the order thiamine (vitamin B1, CAS 70-16-6), pyridoxine (vitamin B6, CAS 65-23-6), nicotinamide (vitamin B3, niacinamide, NAM, CAS 98-92-0), cyanocobolamin (vitamin B12, CAS 68-19-9; spelling as printed in the document) and riboflavin …
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Thiamine (Vitamin B1)CAS number 70-16-6
Pyridoxine (Vitamin B6)CAS number 65-23-6
Nicotinamide (Vitamin B3, Niacinamide)CAS number 98-92-0
Cyanocobolamin (Vitamin B12)CAS number 68-19-9
Riboflavin (Vitamin B2)CAS number 83-88-5
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WESSLING Hungary Kft.
Hungarian branch of the WESSLING group of analytical and consulting laboratories. It provides accredited food, environmental and product testing, including vitamin analysis in food and dietary supplements.
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