Stevia rebaudiana Bertoni is a plant used for its sweet taste. It includes glycosides, which are used as sweeteners in the food industry.
ABSTRACT
Fast isocratic HPLC method for the separation of steviol glycosides from Stevia rebaudiana Bertoni, used as sweeteners in the food industry. The separation is performed on an ASTRA(R) C18-HE column, 3 um, 150 mm x 4.6 mm, part number AST-5732-IK46. Mobile phase: acetonitrile + 0.1% formic acid : water (32 % : 68 % v/v), isocratic elution. Flow rate 1.6 mL/min, temperature 65 C, UV detection at 210 nm; the injection volume is not stated in the document. Analytes are 1 rebaudioside D, 2 rebaudioside A and 3 stevioside; no CAS numbers are given in the note. The single chromatogram shows the standard on the ASTRA(R) C18-HE column, with all peaks eluting within roughly 4.5 minutes (rebaudioside D at about 1.7 min, rebaudioside A at about 4.1 min and stevioside at about 4.4 min) after…
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Rebaudioside DCAS number not available
Rebaudioside ACAS number not available
SteviosideCAS number not available
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