Lutein in food supplements
INTRODUCTION
Lutein and Zeaxanthin are carotenoids used in various dietary supplements. The task of this application was to develop a method that replaces the dichloromethane mobile phase with non-chlorinated solvents.
ABSTRACT
Isocratic HPLC/UV method for the separation of lutein (CAS 127-40-2) and zeaxanthin (CAS 144-68-3) from food supplements, replacing dichloromethane in the mobile phase with non-chlorinated solvents, on an ASTRA® C18-HE, 5 µm column (150 mm × 4.6 mm, AST-5732-LK46). Isocratic elution with ACN/Milli-Q water 90/10 (v/v) at 1.0 ml/min and 30 °C, injection volume 10 µl, UV detection at 450 nm. Both carotenoids are resolved within about 17 minutes.
Industries
Food & Beverage
Category
HPLC columns
Product
Astra C18-HE
Part number
AST-5732-LK46
Analytes
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- Lutein CAS number 127-40-2
- Zeaxanthin CAS number 144-68-3
