Dissolution profile pyridoxine and benfotiamine
INTRODUCTION
The development of the HPLC method for vitamin mixtures in drug products is always challenging, taking into account the requirements of analytical methods in pharmaceutical production. Shown below is a chromatogram of dissolution profile of tablets that contain a mixture of pyridoxine hydrochloride and benfotiamine. ARION® Polar C18 column provides a perfect resolution factor and peaks symmetry in a long sequence of analysis.
ABSTRACT
HPLC/UV method for the dissolution profile of tablets containing pyridoxine hydrochloride (Vitamin B6, CAS 58-56-0) and benfotiamine (CAS 22457-89-2), on an ARION® Polar C18, 3.0 µm column (50 mm × 4.6 mm, ARI-5721-IG46). Gradient elution with A: 50 mM KH2PO4 + 0.3 ml 85% H3PO4 (pH 3.0) and B: ACN:A 160/840 (v/v) at 1.6 ml/min and 22 °C, injection volume 10 µl, UV detection at 222 nm. Both vitamins are resolved within about 5 minutes with excellent peak symmetry.
Industries
Pharma & Veterinary Drugs
Category
HPLC columns
Product
Arion Polar C18
Part number
ARI-5721-IG46
Analytes
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- Pyridoxine hydrochloride (Vitamin B6) CAS number 58-56-0
- Benfotiamine CAS number 22457-89-2
