Fatty acids are carboxylic acids with a long side carbon chain typically found in lipids. These acids differ by the number of carbon atoms in the chain and the number of double bonds in the chain. According to the number of double bonds we distinguish saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA). Trans fatty acids are unsaturated fatty acids in which at least one double bond is in the trans position. Capilary column LION™ LN-FAME was designed to provide the required polarity by the high-cyano propyl phase (G48). In this application note you can see a fast, robust and reproducible baseline separation of the 37 most common FAMEs.
ABSTRACT
GC/FID method for the baseline separation of the 37 most common fatty acid methyl esters (FAME) on a LION™ LN-FAME capillary column (30 m × 0.25 mm × 0.20 µm, LNI-5777-FE30), whose high-cyanopropyl phase (G48) provides the required polarity. Cold needle injection of 1 µl (air lock 1 µl), injector 240 °C, S/SL mode with split ratio 10:1, hydrogen carrier gas at constant flow 1 ml/min. Oven program: 60 °C hold 2 min; 15 °C/min to 140 °C; 3 °C/min to 160 °C hold 5 min; 3 °C/min to 190 °C; 25 °C/min to 240 °C hold 1 min. FID detection at 240 °C. Sample: Supelco 37 FAME mix in DCM (1:10). All 37 esters elute between 2.35 min (C4:0) and 30.79 min (C22:6), including the critical pairs C18:1 trans 9 / cis 9 and C18:2 trans 9,12 / cis 9,12. Developed by the University of Technology Brno,…
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros elementum tristique. Duis cursus, mi quis viverra ornare, eros dolor interdum nulla. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros.w
Butanoic Acid Methyl Ester (C4:0)CAS number not available
Hexanoic Acid Methyl Ester (C6:0)CAS number not available
Octanoic Acid Methyl Ester (C8:0)CAS number not available
Decanoic Acid Methyl Ester (C10:0)CAS number not available
Undecanoic Acid Methyl Ester (C11:0)CAS number not available
Dodecanoic Acid Methyl Ester (C12:0)CAS number not available
Tridecanoic Acid Methyl Ester (C13:0)CAS number not available
Myristic Acid Methyl Ester (C14:0)CAS number not available
Myristoleic Acid Methyl Ester (C14:1 cis 9)CAS number not available
Pentadecanoic Acid Methyl Ester (C15:0)CAS number not available
Partners
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros elementum tristique. Duis cursus, mi quis viverra ornare, eros dolor interdum nulla. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros.w
Brno University of Technology, Faculty of Chemistry
Faculty of Chemistry of the Brno University of Technology (Czech Republic). Named in ARION/LION application notes as the developer of the LION(TM) LN-FAME GC application for the separation of 37 fatty acid methyl esters.
Try&Buy
Try a product on your own instrument before you buy it. Fill in the form below and we will get back to you.
Application Request
Haven't found a suitable application for your analysis? Tell us what you need and our specialists will develop one for you.
We value your privacy
We and our digital partners use cookies on this website. Some of them are necessary for the functionality of the website, but you can decide on the following cookies yourself.
Settings
Decline all
Accept all
Necessary/functional
It would not be possible to operate the website without these cookies. They include, for example, cookies for storing selected settings or remembering logins.
Always active
Analytics
These cookies are used to measure and analyze traffic to our website (number of visitors, pages viewed, average browsing time, etc.). By consent, you will allow us to obtain data on how you use our site.
Advertisement
They are used for the purposes of advertisements displayed on third-party websites, including social networks and contextual advertising. They are tailored to your preferences and help us measure the effectiveness of our advertising campaigns. If you disable them, your ad will continue to show as you browse, but it will not be tailored to you and will be less relevant to you.
