Fatty acids are carboxylic acids with a long side carbon chain typically found in lipids. These acids differ by the number of carbon atoms in the chain and the number of double bonds in the chain. According to the number of double bonds we distinguish saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA). Trans fatty acids are unsaturated fatty acids in which at least one double bond is in the trans position. Capilary column LION™ LN-FAME was designed to provide the required polarity by the high-cyano propyl phase (G48). In this application note you can see a fast, robust and reproducible baseline separation of the 37 most common FAMEs.
ABSTRACT
GC/FID method for the baseline separation of the 37 most common fatty acid methyl esters (FAME) on a LION™ LN-FAME capillary column (30 m × 0.25 mm × 0.20 µm, LNI-5777-FE30), whose high-cyanopropyl phase (G48) provides the required polarity. Cold needle injection of 1 µl (air lock 1 µl), injector 240 °C, S/SL mode with split ratio 10:1, hydrogen carrier gas at constant flow 1 ml/min. Oven program: 60 °C hold 2 min; 15 °C/min to 140 °C; 3 °C/min to 160 °C hold 5 min; 3 °C/min to 190 °C; 25 °C/min to 240 °C hold 1 min. FID detection at 240 °C. Sample: Supelco 37 FAME mix in DCM (1:10). All 37 esters elute between 2.35 min (C4:0) and 30.79 min (C22:6), including the critical pairs C18:1 trans 9 / cis 9 and C18:2 trans 9,12 / cis 9,12. Developed by the University of Technology Brno,…
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γ-Linolenic Acid Methyl Ester (C18:3 cis 6,9,12)CAS number not available
α-Linolenic Acid Methyl Ester (C18:3 cis 9,12,15)CAS number not available
Arachidic Acid Methyl Ester (C20:0)CAS number not available
cis-11-Eicosenoic Acid Methyl Ester (C20:1 cis 11)CAS number not available
cis-11,14-Eicosadienoic Acid Methyl Ester (C20:2 cis 11,14)CAS number not available
Heneicosanoic Acid Methyl Ester (C21:0)CAS number not available
cis-8,11,14-Eicosatrienoic Acid Methyl Ester (C20:3 cis 8,11,14)CAS number not available
Arachidonic Acid Methyl Ester (C20:4 cis 5,8,11,14)CAS number not available
cis-11,14,17-Eicosatrienoic Acid Methyl Ester (C20:3 cis 11,14,17)CAS number not available
Behenic Acid Methyl Ester (C22:0)CAS number not available
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Brno University of Technology, Faculty of Chemistry
Faculty of Chemistry of the Brno University of Technology (Czech Republic). Named in ARION/LION application notes as the developer of the LION(TM) LN-FAME GC application for the separation of 37 fatty acid methyl esters.
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