Control of the profile of fatty acids in blood samples is actual due to the role of fatty acids as biomarkers in the pathogenesis of atherosclerosis. Capillary Column LION™ LN-FAME HT GC provides separation of the 40 fatty acids with excellent reproducibility and robustness.
ABSTRACT
GC/FID profiling of 40 fatty acids in blood samples, where the fatty acid profile serves as a biomarker in the pathogenesis of atherosclerosis, on a LION™ LN-FAME HT GC capillary column (60 m × 0.25 mm × 0.2 µm, LNI-5881-FE60). Injection temperature 260 °C, S/SL injection mode with split ratio 12:1, hydrogen carrier gas at constant pressure 80 kPa (40 mL/min). Oven program: 40 °C hold 0 min; 15 °C/min to 160 °C; 50 °C/min to 200 °C hold 5 min; 3 °C/min to 180 °C; 0.5 °C/min to 200 °C; 10 °C/min to 230 °C. FID detection at 260 °C. Sample: GLC 566 (Nu-CHEK, prep.) mix in heptane, total concentration 2.5 mg/mL. All 40 fatty acids elute between 8.81 min (FA 8:0) and 54.67 min (FA 22:6n-3), including the closely eluting pairs FA 18:1n-9 / FA 18:1n-7 and FA 20:3n-3 / FA 20:4n-6. No…
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Charles University, First Faculty of Medicine
First Faculty of Medicine of Charles University, Prague (Czech Republic). Named as the developer of the LION(TM) LN-FAME HT GC application for the profiling of 40 fatty acids in blood samples.
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