Control of the profile of fatty acids in blood samples is actual due to the role of fatty acids as biomarkers in the pathogenesis of atherosclerosis. Capillary Column LION™ LN-FAME HT GC provides separation of the 40 fatty acids with excellent reproducibility and robustness.
ABSTRACT
GC/FID profiling of 40 fatty acids in blood samples, where the fatty acid profile serves as a biomarker in the pathogenesis of atherosclerosis, on a LION™ LN-FAME HT GC capillary column (60 m × 0.25 mm × 0.2 µm, LNI-5881-FE60). Injection temperature 260 °C, S/SL injection mode with split ratio 12:1, hydrogen carrier gas at constant pressure 80 kPa (40 mL/min). Oven program: 40 °C hold 0 min; 15 °C/min to 160 °C; 50 °C/min to 200 °C hold 5 min; 3 °C/min to 180 °C; 0.5 °C/min to 200 °C; 10 °C/min to 230 °C. FID detection at 260 °C. Sample: GLC 566 (Nu-CHEK, prep.) mix in heptane, total concentration 2.5 mg/mL. All 40 fatty acids elute between 8.81 min (FA 8:0) and 54.67 min (FA 22:6n-3), including the closely eluting pairs FA 18:1n-9 / FA 18:1n-7 and FA 20:3n-3 / FA 20:4n-6. No…
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros elementum tristique. Duis cursus, mi quis viverra ornare, eros dolor interdum nulla. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros.w
FA 15:0CAS number not available
FA 16:0CAS number not available
FA 16:1n-7CAS number not available
FA 17:0CAS number not available
FA 17:1n-7CAS number not available
FA 18:0CAS number not available
FA 18:1n-9CAS number not available
FA 18:1n-7CAS number not available
FA 19:0CAS number not available
FA 18:2n-6CAS number not available
Partners
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros elementum tristique. Duis cursus, mi quis viverra ornare, eros dolor interdum nulla. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros.w
Charles University, First Faculty of Medicine
First Faculty of Medicine of Charles University, Prague (Czech Republic). Named as the developer of the LION(TM) LN-FAME HT GC application for the profiling of 40 fatty acids in blood samples.
Try&Buy
Try a product on your own instrument before you buy it. Fill in the form below and we will get back to you.
Application Request
Haven't found a suitable application for your analysis? Tell us what you need and our specialists will develop one for you.
We value your privacy
We and our digital partners use cookies on this website. Some of them are necessary for the functionality of the website, but you can decide on the following cookies yourself.
Settings
Decline all
Accept all
Necessary/functional
It would not be possible to operate the website without these cookies. They include, for example, cookies for storing selected settings or remembering logins.
Always active
Analytics
These cookies are used to measure and analyze traffic to our website (number of visitors, pages viewed, average browsing time, etc.). By consent, you will allow us to obtain data on how you use our site.
Advertisement
They are used for the purposes of advertisements displayed on third-party websites, including social networks and contextual advertising. They are tailored to your preferences and help us measure the effectiveness of our advertising campaigns. If you disable them, your ad will continue to show as you browse, but it will not be tailored to you and will be less relevant to you.
