Food supplements
INTRODUCTION
Food supplements are now trending worldwide. Concentrated sources of nutrients, vitamins and aminoacids are used to support the normal nutritional balance and healthy lifestyle. This application shows the simultaneous determination of cAMP, phenylalanine, tryptophan, FAD, malic and citric acids by accurate HPLC method.
ABSTRACT
Gradient HPLC/TOF-MS method for the simultaneous determination of six food supplement components – malic acid (CAS 6915-15-7), citric acid (CAS 77-92-9), cAMP (CAS 60-92-4), L-phenylalanine (CAS 63-91-2), L-tryptophan (CAS 73-22-3) and flavin adenine dinucleotide (CAS 146-14-5) – on an ARION® Polar C18, 5.0 µm column (250 mm × 4.6 mm, ARI-5721-LM46). Gradient elution with A: ACN and B: 5 mM formic acid at 0.25 mL/min and 30 °C, injection volume 3 µL, TOF MS detection. All six analytes are resolved within about 11 minutes.
Industries
Food & Beverage
Category
HPLC columns
Product
Arion Polar C18
Part number
ARI-5721-LM46
Analytes
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- Malic acid CAS number 6915-15-7
- Citric acid CAS number 77-92-9
- cAMP CAS number 60-92-4
- L-Phenylalanine CAS number 63-91-2
- L-Tryptophan CAS number 73-22-3
- Flavin adenine dinucleotide (FAD) CAS number 146-14-5
