Dihydroprogesterone
INTRODUCTION
Dihydrotestosterone (DHT) is an androgenic hormone synthesized primarily in prostate. DHT plays a primary role in the formation of the male genitalia during embryogenesis and it acts as the primary androgen in the prostate and hair follicles in adulthood. In medicine it is used mainly in the treatment of low testosterone levels. ARION® Biphenyl column allows to determine DHT with sufficient tailing factor and resolution coefficient in the complex mixture of excipients in finished dosage form.
ABSTRACT
LC-MS/MS method for the determination of dihydrotestosterone (DHT, CAS 521-18-6) in a finished dosage form, on an ARION® Biphenyl, 3 µm column (50 mm × 2.1 mm, ARI-5868-IG21). Gradient elution with A: water + 0.1% formic acid and B: ACN + 0.1% formic acid at 0.3–0.4 mL/min and 25 °C, injection volume 8 µL, MS/MS detection with positive ESI, nitrogen collision gas and MRM transitions 291.1 → 255 and 291.1 → 159. DHT elutes at about 4.4 minutes with a better peak shape than on the two compared core-shell columns (Competitor P and Competitor C).
Industries
Pharma & Veterinary Drugs
Category
HPLC columns
Product
Arion Biphenyl
Part number
ARI-5868-IG21
Analytes
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- Dihydrotestosterone (DHT) CAS number 521-18-6
