A quantitative determination of steroids is a valuable tool in a clinical practice in diagnostic of the steroid-related diseases. This method shows the simultanious analysis of wide panel of steroids by sensitive LC-MS/MS method.
ABSTRACT
Quantitative LC-MS/MS determination of a wide panel of 22 steroids for the diagnosis of steroid-related diseases, on an ARION® C18 Plus, 2.2 µm column (100 mm × 3.0 mm, ARI-5720-EI30). Gradient elution with A: water + 0.1% formic acid and B: methanol + 0.1% formic acid at 600 µL/min and 40 °C, injection volume 20 µL, MS/MS detection with a quantifier and qualifier transition for each analyte. All steroids elute between about 5.1 and 6.9 minutes. This application was developed by the Institute of Endocrinology, Prague.
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Institute of Endocrinology, Prague
Czech research institute in Prague specialising in endocrinology. Named in APP NOTE 488 as the developer of the LC-MS/MS method for the simultaneous analysis of a wide panel of steroids.
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