Amino acids plays a valuable role in spermatogenesis, fertilization success and impacts on survival of juvenile fish. This application shows amino acids profile in Tilapia sperm tested by LC-MS MetAmino® kit. The data give better understanding of adjustment in feeding ration needed to increase reproductive function and population of food fish species.
ABSTRACT
LC-MS profiling of amino acids in Tilapia sperm using the MetAmino® kit. Sample preparation: precipitation medium (PM, 80 µL) was added to 20 µL of the Tilapia sperm sample, the sample was placed in an ultrasonic bath for 5 minutes at room temperature and centrifuged at 7000 rpm for 5 min at 4 °C; the supernatant (25 µL) was transferred to a new tube for the Metamino derivatization, IS (10 µL) was added and the protocol was continued with the addition of CTS and RDS according to the general protocol scheme referenced in the note. The chromatographic conditions are given only as an LC-MS method according to the same general protocol scheme, so no column, dimensions, part number, mobile phase, gradient, flow rate, temperature, injection volume or detector settings are specified.…
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Arginine (ARG)CAS number not available
Histidine (HIS)CAS number not available
Lysine (LYS)CAS number not available
Tyrosine (TYR)CAS number not available
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Biology Centre CAS, České Budějovice
Biology Centre of the Czech Academy of Sciences, České Budějovice (Czech Republic). Named as the developer of the SpeExtra(TM) MSPE application for the pre-purification of insect adipokinetic hormones.
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