Amino acids play an important role in the life processes of human organism. They are using as building blocks for proteins and regulate a key metabolic pathways. Many kinds of mental disorders, liver and immune system diseases are related to metabolic disorders of amino acids. AAs profile, determined in human blood serum, is used for an early diagnostic of various diseases. This application shows amino acids profile in healthy human blood serum tested by LC-MS MetAmino® kit.
ABSTRACT
LC-MS profiling of amino acids in healthy human blood serum using the MetAmino® kit. Sample preparation is given only as being according to the general protocol scheme referenced in the note, and the chromatographic conditions only as an LC-MS method according to the same scheme, so no column, dimensions, part number, mobile phase, gradient, flow rate, temperature, injection volume or detector settings are specified. The note shows two mass extracted chromatograms: the standard mixture with 5 nmol of SD1 and SD2 according to the kit manual, with labelled peaks including cotinine, ARG, IS = HARG, GLN, CIT, HLY/1MHIS/PHP/SER/3MHIS/ASN, HYP/GLY/GPR, THR, ALA, GABA/SAR, BAIBA, ABA, PRO, MET with IS = MET-D3, TPR, VAL, ORN/TRP, HIS/LYS, PHE/LEU/ASP, GLU/ILE, AAA with IS = HPHE, APA,…
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Glutamic acid (GLU)CAS number not available
Isoleucine (ILE)CAS number not available
Tyrosine (TYR)CAS number not available
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Biology Centre CAS, České Budějovice
Biology Centre of the Czech Academy of Sciences, České Budějovice (Czech Republic). Named as the developer of the SpeExtra(TM) MSPE application for the pre-purification of insect adipokinetic hormones.
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