Vitellogenins, glycolipophosphoproteins mostly known and well-characterized as precursors of yolk proteins, are involved in reproduction in the majority of oviparous animals. In insects, vitellogenins are typically synthesized in the fat body, from where they are transported via the haemolymph into growing oocytes. The honey bees are no exception: vitellogenin (mw about 200 kDa) is known to play crucial roles in bee reproduction, but its involvement appears to be multifunctional, since vitellogenin influences oxidative stress, nutrition, immunity, and honey bee longevity.
ABSTRACT
Reversed-phase HPLC purification of vitellogenin (VTG) from honey bee haemolymph on an ARION® BIO C4 5 µm column, 250 mm × 4.6 mm, part number ARI-5846-LM46. Mobile phase A is 0.11% TFA in water, mobile phase B is 0.1% TFA in 60% ACN. Gradient elution (time / A% / B% / flow rate in mL/min): 0 / 90 / 10 / 1.0, 2 / 90 / 10 / 1.0, 20 / 0 / 100 / 1.0, 30 / 0 / 100 / 1.0. Injection volume 3 µL, UV detection at 215 nm and 280 nm. Single analyte: vitellogenin (VTG), no CAS number given; the introduction states its molecular weight as about 200 kDa. The note shows two elution profiles of bee queen haemolymph on the same column, one detected at 215 nm and one at 280 nm; in both the peak marked 1 and assigned to VTG elutes at about 21.5 min as the largest peak of the profile, following a…
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Vitellogenin (VTG)CAS number not available
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Biology Centre CAS, České Budějovice
Biology Centre of the Czech Academy of Sciences, České Budějovice (Czech Republic). Named as the developer of the SpeExtra(TM) MSPE application for the pre-purification of insect adipokinetic hormones.
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