CIT_MIT preservatives determination
INTRODUCTION
CIT/MIT biocides are widely used as preservatives in variety of applications, which include household products, personal care products and polymer materials. Apart of their antimicrobial activity, CIT/MIT preservatives may cause allergy reactions. Due to this reason, the preservatives additives should be used at the lowest possible concentration and strictly controlled. This application shows the determination of CIT/MIT preservative in rhamnolipids production. ARION® C8 column shows good resolution and peaks symmetry for CIT/MIT components at low concentrations.
ABSTRACT
HPLC determination of the CIT/MIT preservative system in rhamnolipids production on an ARION® C8 5 µm column, 250 mm × 4.6 mm, part number ARI-5734-LM46. Mobile phase A is 0.1% H3PO4 in water and mobile phase B is methanol : THF 95/5 (v/v); the note states gradient elution but does not print the gradient programme. Flow rate 1.0 mL/min, injection volume 10 µL, column temperature 30 °C, DAD detection at 273 nm. Analytes: 1. MIT (methylisothiazolinone), CAS number 2682-20-4, and 2. CIT (methylchloroisothiazolinone), CAS number 26172-55-4. The note shows a chromatogram of the test solution on the ARION® C8 column with MIT at about 4.4 min and CIT at about 12.9 min, both symmetrical and well resolved at low concentrations, together with the structural formulae of…
Industries
Category
HPLC columns
Product
Arion C8
Part number
ARI-5734-LM46
Analytes
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- MIT (Methylisothiazolinone) CAS number 2682-20-4
- CIT (Methylchloroisothiazolinone) CAS number 26172-55-4
