5-Hydroxymethylfurfural (5-HMF) is formed from fructose or glucose by the heat treatment of food. HMF and its derivates/metabolites are genotoxic, mutagenic and may be carcinogenic, which is why HMF is analysed in various food matrices, such as in fruit and vegetable products, instant coffee and honey.
ABSTRACT
Determination of 5-hydroxymethylfurfural in syrup on an ARION® Plus C18, 5.0 µm column (250 mm × 4.6 mm, ARI-5720-LM46). Isocratic elution with methanol/water 10/90 (v/v) at 1.5 ml/min and 30 °C, injection volume 5.0 µl, UV detection at 285 nm. The analyte elutes as a single sharp peak at about 6 minutes; a comparison with a core-shell column (Competitor K) is included.
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HydroxymethylfurfuralCAS number 170678-36-1
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