Flumetrin and fluvalinate are pyrethroid insecticides used to control mites in honeybee colonies. The treatment by these insecticides should be strictly controlled due to its toxic effects on health of honeybees. The described method allows to determine flumetrin and fluvalinate in honey and related biological samples matrixes.
ABSTRACT
HPLC determination of the pyrethroid insecticides fluvalinate and flumethrin in honey and related biological sample matrices on a column printed in the note as ASTRA® C18-HE C18, 3.0 µm, 150 mm × 4.6 mm, part number AST-5732-IK46. Mobile phase ACN : water 90/10 (v/v), isocratic elution. Flow rate 1.5 mL/min, column temperature 25 °C, PDA detection at 255 nm. Analytes: 1. fluvalinate, CAS number 69409-94-5, and 2. flumethrin, CAS number 69770-45-2. The note shows a chromatogram of the test solution on the ASTRA® column, with fluvalinate at about 4.2 min and flumethrin at about 5.5 min, both as sharp, well separated peaks after an early matrix peak at about 2.1 min, and the structural formulae of fluvalinate and flumethrin. No comparison with competitive columns is presented. The…
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FluvalinateCAS number 69409-94-5
FlumethrinCAS number 69770-45-2
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Beekeeping Research Institute
Czech research institute focused on honeybee health, breeding and beekeeping technology. It develops and produces veterinary preparations against bee parasites, including the amitraz-based Varidol solution.
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