HPLC-RID separation of sucrose, glucose and fructose on an ASTRA® Sugar Ca(S) 10 µm column, 300 mm × 8 mm, part number AST-5906-VN80. Mobile phase 100% H2O, column temperature 70 °C, injection volume 20 µL, refractive index detection at 35 °C. The conditions table does not state a flow rate; the two chromatograms are captioned as recorded at 0.5 mL/min and at 1.5 mL/min. Analytes: 1. sucrose, CAS number 57-50-1, 2. glucose, CAS number 2280-44-6, 3. fructose, CAS number 57-48-7. The note shows the analysis on the ASTRA® Sugar Ca(S) column at 0.5 mL/min, with sucrose at about 10 min, glucose at about 11.5 min and fructose at about 14 min, and the same separation at 1.5 mL/min, where the three sugars elute between about 4 and 6 min with the run compressed accordingly and still…