LC-MS/MS determination of 21 pyrrolizidine alkaloids and their N-oxides in herbal tea – among them lycopsamine, intermedine, europine, heliotrine, retrorsine, seneciphylline, senecivernine, senecionine, senkirkine, echimidine and lasiocarpine together with the corresponding N-oxides – on an ARION® Plus C18, 2.2 µm column (100 mm × 2.1 mm, ARI-5720-EI21). Isocratic elution with 10 mM ammonium carbonate in water/ACN (LC-MS grade) at 0.4 mL/min and 40 °C, injection volume 5 µL. Retention times run from 4.12 min (lycopsamine N-oxide) to 12.01 min (lasiocarpine); each analyte is confirmed by three MRM transitions.