This application shows the method of analysis of the main photosynthetics pigments in extracts of Hibiscus leaves and extract from cells of the algae Emiliania huxleyi. ASTRA® C18-HE column allows to achieve good separation of the pigments, which is always challenging.
ABSTRACT
Gradient HPLC/UV analysis of the main photosynthetic pigments in extracts of Hibiscus leaves and of cells of the algae Emiliania huxleyi, on an ASTRA® C18, 5 µm column (250 mm × 4.6 mm, AST-5732-LM46). Mobile phase A: methanol : 0.5M ammonium acetate 80/20 (v/v), B: ACN : water 90/10 (v/v), C: ethyl acetate, gradient elution at 30 °C, max. pressure 145 bar, UV detection at 435 nm. The Hibiscus extract gives neoxanthin, violaxanthin, lutein, chlorophyll b, chlorophyll a and β-carotene; the Emiliania huxleyi extract gives chlorophyll c2, chlorophyll c3, fucoxanthin, 19'-hexanoyloxyfucoxanthin, diadinoxanthin, chlorophyll a and β-carotene, all within about 25 minutes. Note: this column does not solve the separation of lutein and zeaxanthin. Author of this application: RNDr. Radek…
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NeoxanthinCAS number 14660-91-4
ViolaxanthinCAS number 126-29-4
LuteinCAS number 127-40-2
Chlorophyll bCAS number 519-62-0
Chlorophyll aCAS number 479-61-8
β-CaroteneCAS number 7235-40-7
Chlorophyll c2CAS number 27736-03-4
Chlorophyll c3CAS number 111308-93-1
FucoxanthinCAS number 3351-86-8
19'-HexanoyloxyfucoxanthinCAS number 60147-85-5
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University of South Bohemia in České Budějovice, Faculty of Science
Faculty of Science of the University of South Bohemia in České Budějovice (Czech Republic). Named as the developer of the ARION(R) HPLC applications for caffeine metabolites in urine, atorvastatin photodegradation products and the degradation assessment of diltiazem.
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