Amino acids analysis in urine is excellent tool for identification of gastrointestinal and renal dysfunctions. It also may provide supportive information towards nutritional deficiencies of vitamins and minerals. MetAmino® kit provides a rapid, simple and precise method for the quantitative determination of wide panel of amino acids in urine. The test reflects recent diet and may be used to assess the impact of dietary changes.
ABSTRACT
LC-MS determination of a wide panel of amino acids in human urine using the MetAmino® kit. Sample preparation: 10 µL of IS was added to 25 µL of unprecipitated fresh urine sample and the protocol was continued with the addition of CTS and RDS according to the general protocol scheme referenced in the note. The chromatographic conditions are given only as an LC-MS method according to the same general protocol scheme, so no column, dimensions, part number, mobile phase, gradient, flow rate, temperature, injection volume or detector settings are specified. The note shows two mass extracted chromatograms: the standard mixture with 5 nmol of SD1 and SD2 according to the kit manual, with labelled peaks including cotinine, ARG, IS = HARG, GLN, CIT, HLY/1MHIS/PHP/SER/3MHIS/ASN, HYP/GLY…
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Glutamine (GLN)CAS number not available
Asparagine (ASN)CAS number not available
Serine (SER)CAS number not available
1-Methylhistidine (1MHIS)CAS number not available
PHPCAS number not available
3-Methylhistidine (3MHIS)CAS number not available
Glycine (GLY)CAS number not available
GPRCAS number not available
Threonine (THR)CAS number not available
Alanine (ALA)CAS number not available
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Biology Centre CAS, České Budějovice
Biology Centre of the Czech Academy of Sciences, České Budějovice (Czech Republic). Named as the developer of the SpeExtra(TM) MSPE application for the pre-purification of insect adipokinetic hormones.
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