PFASs are a group of synthetic organofluorine chemical compounds used in the production of fluoropolymer coatings and products such as furniture, adhesives, personal care products, food packaging, mobile phone screens, etc. Many PFASs pose health and environmental concerns because they are classified as persistent organic pollutants. The long-chain PFASs are due to higher bioaccumulative potential frequently detected in living organisms. This application shows a robust method of extraction and determination of the PFAS compounds in fish biota samples, which can be a relevant exposure pathway for humans.
MSPE with 15 mg of C18 stationary phase and nylon frits was used to analyze 14 PFAS compounds in fish tissue samples. The MSPE sample preparation approach was compared with the reference extraction method without SPE based on Grabicova et al. 2018 [1]. Both sets of samples were fortified to 1 ng/g of PFAS. The MSPE method enhances the response of most of the analytes between 80–130 % (Fig. 1). Although sample preparation is more demanding than the reference method, the MSPE offers relevant signal improvement.
ABSTRACT
Determination of 14 long-chain PFAS compounds in fish tissue by LC-HRMS after micro solid phase extraction (MSPE). MSPE method: Micro Spin SpeExtra™ MSPE C18 column with 0.22 µm nylon membrane, 15 mg, part number MSP-5876-AA07; homogenization on a TissueLyser II (Quiagen, Germany) at 1800 min-1 for 10 min; extraction with ACN + 0.1% FA; centrifugation on a Mini spin (Eppendorf, Germany) at 4000 rpm for 2 min; reconstitution in DDW + 0.1% FA; dissolution in an ultrasonic bath for 5 min; MSPE activation with DDW + MeOH (50:50); MSPE equilibration with DDW + 0.1% FA; MSPE extraction of analytes from 500 µL of sample; MSPE elution of analytes with 200 µL of ACN + 0.1% FA. The note recommends the use of PFAS suitable vials and caps, for example CHS silicone/PP septa and PP caps. LC…
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Faculty of Fisheries and Protection of Waters
Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, Laboratory of Environmental Chemistry and Biochemistry. Named in APP NOTE 533 as the co-operating partner for the MSPE and LC-HRMS determination of long-chain PFASs in fish biota.
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